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Rapport entre le changement de volume cellulaire des spermatozoïdes de poisson et leur cryorésistance.

Relationship between the changes in cellular volume of fish spermatozoa and their cryoresistance.

Auteurs : DZUBA B. B., KOPEIKA E. F.

Type d'article : Article

Résumé

The authors investigated the hypothesis that the spermatozoa of marine fish are more resistant than freshwater species to the dynamic changes in osmotic pressure that occur during the process of cryopreservation. They showed that while the spermatozoa of marine fish can be successfully activated across a wide range of osmotic pressures, those of the freshwater species only survive activation within a more restricted range. After freeze-thawing, up to 30% of motile cells were found in silver carp samples, while up to 90% of motile cells were observed in samples from the haarder (Mugil soiuy B). Haarder spermatozoa showed no change in cell volume after dilution in activating or cryoprotective media, while silver carp spermatozoa responded by swelling and eventual cell disruption. The authors suggested that the differences in cryoresistance between silver carp and haarder spermatozoa may be determined by the ability to preserve cellular volume under non-isotonic conditions.

Détails

  • Titre original : Relationship between the changes in cellular volume of fish spermatozoa and their cryoresistance.
  • Identifiant de la fiche : 2003-3026
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 6
  • Date d'édition : 11/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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