Partager

Document IIF

Technologies cryogéniques pour la microscopie électronique.

Cryogenic technologies for electron microscopy.

Numéro : 0064

Auteurs : URBAN P., BRAUNER T., KRUTIL V., HANZELKA P., HRUBANOVA K., KRZYZANEK V.

Résumé

Modern electron cryomicroscopes (cryo-EMs) are widely used in structural biology and materials science, as samples at cryogenic temperatures exhibit significantly higher resistance to radiation damage from electron or ion beam exposure. Cryo-EM typically uses liquid nitrogen (LN2) with a normal boiling point of 77 K and liquid helium (LHe) with a boiling point of 4.2 K, for sample cooling. The simplest cryo-EM cooling systems use either bath cooling or flow cooling methods. We present an overview of our cryogenic technologies developed for both cooling methods, enabling the conversion of existing room-temperature EM to cryo-EM, including specific implementations of these conversions. These technologies include a nitrogen Dewar vessel with a cooled copper finger, a LHe/LN2 flow cryostat containing two heat exchangers, a low-loss LHe/LN2 vacuum-insulated transfer line with flow rate control, a thermally insulating pad with high mechanical stiffness, and a cryogenic sample holder equipped with ten spring-loaded electrical contacts.

Documents disponibles

Format PDF

Pages : 7 p.

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Cryogenic technologies for electron microscopy.
  • Identifiant de la fiche : 30033900
  • Langues : Anglais
  • Sujet : Technologie
  • Source : Cryogenics 2025. Proceedings of the 18th IIR International Conference on Cryogenics, Prague, Czech Republic, 7-11 April 2025.
  • Date d'édition : 07/04/2025
  • DOI : http://dx.doi.org/10.18462/iir.cryo.2025.0064

Liens


Voir d'autres communications du même compte rendu (51)
Voir le compte rendu de la conférence

Indexation