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Ultrastructure des spermatozoïdes de carpe Cyprinus carpio après refroidissement, dilution et décongélation.

Ultrastructure of carp Cyprinus carpio spermatozoa after cooling, dilution and freeze-thawing.

Auteurs : DROKING S. I., STEIN H., GOVORUKHA T. P.

Type d'article : Article

Résumé

Dilution of the carp spermatozoa with a cryoprotective medium was shown to inflict serious injuries to the fine structures of some spermatozoa. The number of injured spermatozoa increases after cryopreservation. Examination of the freeze-fracture micrographs of the heads of the carp spermatozoa showed that their protoplasmic faces were covered with particles. These particles do not form clusters after cooling the spermatozoa to 5 °C and dilution with cryoprotective medium. The authors observed that the particles form geometrically shaped regular formations on the outer membranes of the carp spermatozoa. On the basis of the results obtained, the authors propose that the high susceptibility of the carp spermatozoa to osmotic exposures is apparently due to some particular features of the fine structures of their membranes.

Détails

  • Titre original : Ultrastructure of carp Cyprinus carpio spermatozoa after cooling, dilution and freeze-thawing.
  • Identifiant de la fiche : 2003-3035
  • Langues : Anglais
  • Source : CryoLetters - vol. 24 - n. 1
  • Date d'édition : 01/2003
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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