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Ultrastructure of carp Cyprinus carpio spermatozoa after cooling, dilution and freeze-thawing.

Author(s) : DROKING S. I., STEIN H., GOVORUKHA T. P.

Type of article: Article

Summary

Dilution of the carp spermatozoa with a cryoprotective medium was shown to inflict serious injuries to the fine structures of some spermatozoa. The number of injured spermatozoa increases after cryopreservation. Examination of the freeze-fracture micrographs of the heads of the carp spermatozoa showed that their protoplasmic faces were covered with particles. These particles do not form clusters after cooling the spermatozoa to 5 °C and dilution with cryoprotective medium. The authors observed that the particles form geometrically shaped regular formations on the outer membranes of the carp spermatozoa. On the basis of the results obtained, the authors propose that the high susceptibility of the carp spermatozoa to osmotic exposures is apparently due to some particular features of the fine structures of their membranes.

Details

  • Original title: Ultrastructure of carp Cyprinus carpio spermatozoa after cooling, dilution and freeze-thawing.
  • Record ID : 2003-3035
  • Languages: English
  • Source: CryoLetters - vol. 24 - n. 1
  • Publication date: 2003/01
  • Document available for consultation in the library of the IIR headquarters only.

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