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Vitrification à basse température et à température élevée en tant que nouvelle approche à la biostabilisation des cellules reproductrices ainsi que des cellules souches.

Low- and high-temperature vitrification as a new approach to biostabilisation of reproductive and progenitor cells.

Auteurs : KATKOV I. I., ISACHENKO V., ISACHENKO E., et al.

Type d'article : Article, Article de la RIF

Résumé

Cells held in a liquid medium undergo processes that cause progressive loss of viability. To prevent such degradation, cells need to be placed in conditions that essentially stop all chemical reactions for the duration of the time of storage. Because intracellular ice formation is lethal to most eukaryotic cells, stable storage of viable cells can be achieved only if intracellular vitrification without ice formation has occurred. This has been done by several methods, including equilibrium (slow) freezing, lyophilization (freeze-drying), and ice-free vitrification at subzero temperatures at moderate-to high cooling rates in the presence of cryoprotectants ('conventional' vitrification). In this paper, the authors discuss the mechanisms of vitrification, and specific aspects, advantages, and pitfalls of the different approaches. Particular emphasis is put on novel methods of cell preservation, such as cryoprotectant-free vitrification of sperm and high-temperature vitrification by air/vacuum drying of progenitor and other nucleated cells.

Documents disponibles

Format PDF

Pages : 346-357

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Low- and high-temperature vitrification as a new approach to biostabilisation of reproductive and progenitor cells.
  • Identifiant de la fiche : 2006-1509
  • Langues : Anglais
  • Source : International Journal of Refrigeration - Revue Internationale du Froid - vol. 29 - n. 3
  • Date d'édition : 05/2006

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