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Altération sublétale pendant la cryoconservation du sperme de la truite arc-en-ciel.

Sublethal damage during cryopreservation of rainbow trout sperm.

Auteurs : CABRITA E., ALVAREZ R., ANEL L., RANA K. J., HERRAEZ M. P.

Type d'article : Article

Résumé

The aim of this study was to evaluate sublethal damage caused during cryopreservation by analyzing the resistance of live spermatozoa to hypoosmotic shock and examining the effect of egg yolk as a membrane stabilizer. The use of egg yolk promoted significantly increased membrane resistance and sperm motility, but a similar increase in fertility was not observed. The low fertility rates achieved with post-thawed semen in all trials could be exacerbated by the use of water as the sperm activator. The data suggest that new extenders containing different membrane stabilizers and motility activators with osmolalities exceeding 150 mOsm/kg are needed to minimize sublethal cryodamage and to improve fertility rates of trout sperm after freezing and thawing.

Détails

  • Titre original : Sublethal damage during cryopreservation of rainbow trout sperm.
  • Identifiant de la fiche : 1999-3916
  • Langues : Anglais
  • Source : Cryobiology - vol. 37 - n. 3
  • Date d'édition : 11/1998

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