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Comportement des tissus biologiques lors de la congélation et la décongélation révélés par l'imagerie en temps réel utilisant la microscopie à foyers convergents à balayage laser.

Three-dimensional microscopic freezing and thawing behaviour of biological tissues revealed by real-time imaging using confocal laser scanning microscopy.

Auteurs : ISHIGURO H., HORIMIZU T.

Type d'article : Article

Résumé

Three-dimensional behaviour of ice crystals and cells during freezing and thawing of biological tissues was investigated microscopically in real time by using a confocal laser scanning microscope (CLSM) and a fluorescent dye. Fresh white meat of chicken was stained in physiological saline, and then frozen and thawed under two different thermal protocols. The CLSM noninvasively produced tomograms of the tissues to clarify the pattern of freezing, the morphology of ice crystals in the tissues, and the interaction between ice crystals and cells. The results were compared and correlated with observed histological changes in the post-thaw tissues. [Reprinted with permission from Elsevier. Copyright, 2008].

Détails

  • Titre original : Three-dimensional microscopic freezing and thawing behaviour of biological tissues revealed by real-time imaging using confocal laser scanning microscopy.
  • Identifiant de la fiche : 2009-0875
  • Langues : Anglais
  • Source : International Journal of Heat and Mass Transfer - vol. 51 - n. 23-24
  • Date d'édition : 11/2008

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