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Cryoconservation d'embryons somatiques d'olives 'Nabali' (Olea europea L.) à l'aide de techniques d'encapsulation-déshydratation et d'encapsulation-vitrification.

Cryopreservation of 'Nabali' olive (Olea europea L.) somatic embryos by encapsulation-dehydration and encapsulation-vitrification.

Auteurs : SHIBLI R. A., JUBOORY K. H. al-

Type d'article : Article

Résumé

Olive (Olea europea L.) somatic embryos were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification. In the encapsulation-dehydration procedure, 48% embryo survival was obtained when bead moisture content was decreased to 21.1% after 4 h dehydration. Preculture of embryos for 4 d in medium containing 0.75 to 1.25 M sucrose produced higher (40 to 34%, respectively) regrowth after cryopreservation. Dehydration of beads for 3 h in PVS2 ensured higher survival (64%) of encapsulated-vitrified and cryopreserved (EVN) somatic embryos. Thermal treatment of embryogenic callus for 1 d at 30 °C was very effective to increase survival of encapsulated-dehydrated and cryopreserved (EDN) (58%) and EVN (68%) embryos. Plantlets produced from control and cryopreserved embryos were phenotypically similar.

Détails

  • Titre original : Cryopreservation of 'Nabali' olive (Olea europea L.) somatic embryos by encapsulation-dehydration and encapsulation-vitrification.
  • Identifiant de la fiche : 2002-0207
  • Langues : Anglais
  • Source : CryoLetters - vol. 21 - n. 6
  • Date d'édition : 11/2000
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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