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Cryopreservation of 'Nabali' olive (Olea europea L.) somatic embryos by encapsulation-dehydration and encapsulation-vitrification.

Author(s) : SHIBLI R. A., JUBOORY K. H. al-

Type of article: Article

Summary

Olive (Olea europea L.) somatic embryos were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification. In the encapsulation-dehydration procedure, 48% embryo survival was obtained when bead moisture content was decreased to 21.1% after 4 h dehydration. Preculture of embryos for 4 d in medium containing 0.75 to 1.25 M sucrose produced higher (40 to 34%, respectively) regrowth after cryopreservation. Dehydration of beads for 3 h in PVS2 ensured higher survival (64%) of encapsulated-vitrified and cryopreserved (EVN) somatic embryos. Thermal treatment of embryogenic callus for 1 d at 30 °C was very effective to increase survival of encapsulated-dehydrated and cryopreserved (EDN) (58%) and EVN (68%) embryos. Plantlets produced from control and cryopreserved embryos were phenotypically similar.

Details

  • Original title: Cryopreservation of 'Nabali' olive (Olea europea L.) somatic embryos by encapsulation-dehydration and encapsulation-vitrification.
  • Record ID : 2002-0207
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 6
  • Publication date: 2000/11
  • Document available for consultation in the library of the IIR headquarters only.

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