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Cryoconservation d'embryons somatiques de pivoines blanches (Paeonia lactiflora Pall.) par séchage à l'air.

Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying.

Auteurs : KIM H. M., SHIN J. H., SOHN J. K.

Type d'article : Article

Résumé

This study was carried out to establish a suitable method for the cryopreservation of somatic embryos of the herbaceous peony. The somatic embryos were obtained from cotyledon and anther cultures on a MS medium supplemented with abscisic acid (ABA) and phenylacetic acid (PAA), respectively. The frequency of somatic embryo formation was the greatest (61%) from the cotyledons cultured on a MS medium supplemented with 1.0 mg/l of ABA. Embryos were also obtained directly from anthers cultured on a MS medium with or without 2.0 mg/l of PAA. For the cryopreservation of peony somatic embryos, the embryos were dried under a stream of sterile air and frozen by immersion in liquid nitrogen. Thawed embryos were germinated into plantlets after placing on a medium containing 0.3 mg/l of gibberellic acid (GA3). The frequency of the post-thaw regrowth of cryopreserved somatic embryos was related to their size and desiccation time, the latter ranging from 0 to 2 h. When the somatic embryos were desiccated for 1 h, the frequency of post-thaw regrowth was greater than 66%. The frequency of post-thaw regrowth of the cryopreserved somatic embryos from anthers and cotyledon tissues was generally high when they were 2-3 mm in size. Desiccation may be a suitable method for the cryopreservation of somatic embryos of the herbaceous peony. [Reprinted with permission from Elsevier. Copyright, 2006].

Détails

  • Titre original : Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying.
  • Identifiant de la fiche : 2006-3222
  • Langues : Anglais
  • Source : Cryobiology - vol. 53 - n. 1
  • Date d'édition : 08/2006

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