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Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying.

Author(s) : KIM H. M., SHIN J. H., SOHN J. K.

Type of article: Article

Summary

This study was carried out to establish a suitable method for the cryopreservation of somatic embryos of the herbaceous peony. The somatic embryos were obtained from cotyledon and anther cultures on a MS medium supplemented with abscisic acid (ABA) and phenylacetic acid (PAA), respectively. The frequency of somatic embryo formation was the greatest (61%) from the cotyledons cultured on a MS medium supplemented with 1.0 mg/l of ABA. Embryos were also obtained directly from anthers cultured on a MS medium with or without 2.0 mg/l of PAA. For the cryopreservation of peony somatic embryos, the embryos were dried under a stream of sterile air and frozen by immersion in liquid nitrogen. Thawed embryos were germinated into plantlets after placing on a medium containing 0.3 mg/l of gibberellic acid (GA3). The frequency of the post-thaw regrowth of cryopreserved somatic embryos was related to their size and desiccation time, the latter ranging from 0 to 2 h. When the somatic embryos were desiccated for 1 h, the frequency of post-thaw regrowth was greater than 66%. The frequency of post-thaw regrowth of the cryopreserved somatic embryos from anthers and cotyledon tissues was generally high when they were 2-3 mm in size. Desiccation may be a suitable method for the cryopreservation of somatic embryos of the herbaceous peony. [Reprinted with permission from Elsevier. Copyright, 2006].

Details

  • Original title: Cryopreservation of somatic embryos of the herbaceous peony (Paeonia lactiflora Pall.) by air drying.
  • Record ID : 2006-3222
  • Languages: English
  • Source: Cryobiology - vol. 53 - n. 1
  • Publication date: 2006/08

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