Cryoconservation de cartilage articulaire de porc : IRM et résultats biochimiques après divers protocoles de congélation.
Cryopreservation of porcine articular cartilage: MRI and biochemical results after different freezing protocols.
Auteurs : LAOUAR L., FISHBEIN K., MCGANN L. E., et al.
Type d'article : Article
Résumé
The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups: 1) phosphate buffered saline (PBS) control; 2) PBS snap frozen in liquid nitrogen; 3) slow-cooled in dimethylsulfoxide(DMSO); and 4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposureto ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetizationtransfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36-72 h in PBS in terms of chondroitin sulfate and hydroxyproline content. [Reprinted with permission from Elsevier. Copyright, 2006].
Détails
- Titre original : Cryopreservation of porcine articular cartilage: MRI and biochemical results after different freezing protocols.
- Identifiant de la fiche : 2007-1308
- Langues : Anglais
- Source : Cryobiology - vol. 54 - n. 1
- Date d'édition : 02/2007
Liens
Voir d'autres articles du même numéro (5)
Voir la source
Indexation
- Thèmes : Influence du froid sur les cellules, tissus et organes
- Mots-clés : Imagerie; Cryoprotecteur; Cartilage; DMSO; Porc; Détection; Cryoconservation
-
Localization of freezing injury in articular ca...
- Auteurs : MULDREW K., HURTIG M., NOVAK K., SCHACHAR N., MCGANN L. E.
- Date : 02/1994
- Langues : Anglais
- Source : Cryobiology - vol. 31 - n. 1
Voir la fiche
-
Dimethyl sulfoxide (DMSO) concentration in fres...
- Auteurs : HU J. F., WOLFINBARGER L. Jr
- Date : 10/1994
- Langues : Anglais
- Source : Cryobiology - vol. 31 - n. 5
Voir la fiche
-
Development of a protocol for the quantitative ...
- Auteurs : ISBELL S. A.
- Date : 03/1997
- Langues : Anglais
- Source : Cryobiology - vol. 34 - n. 2
Voir la fiche
-
Effects of four cryoprotectants in combination ...
- Auteurs : POLLOCK G. A., et al.
- Date : 10/1991
- Langues : Anglais
- Source : Cryobiology - vol. 28 - n. 5
Voir la fiche
-
Effets du cryoprotecteur sur l'expansion thermi...
- Auteurs : YU H., LI D., XU Y., et al.
- Date : 08/2014
- Langues : Chinois
- Source : Journal of Refrigeration - vol. 35 - n. 158
- Formats : PDF
Voir la fiche