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Cryopreservation of porcine articular cartilage: MRI and biochemical results after different freezing protocols.

Author(s) : LAOUAR L., FISHBEIN K., MCGANN L. E., et al.

Type of article: Article

Summary

The objective of this study was to investigate the effects of cryopreservation on the components of articular cartilage (AC) matrix by utilizing magnetic resonance imaging (MRI) and biochemical assessments. Porcine AC (10 mm osteochondral dowels) was collected into four groups: 1) phosphate buffered saline (PBS) control; 2) PBS snap frozen in liquid nitrogen; 3) slow-cooled in dimethylsulfoxide(DMSO); and 4) slow cooled in PBS (in absence of DMSO). MRI results demonstrated three distinct zones in the cartilage. After exposureto ice formation during cryopreservation procedures, alterations in MRI determined matrix fixed charged density and magnetizationtransfer rate were noted. In addition, biochemical assays demonstrated significant alterations in chondroitin sulfate and hydroxyproline content over time without differences in hydration or DNA content. In conclusion, MRI was able to detect some changes in the intact cartilage matrix structure consistent with biochemical assessments after ice formation during cryopreservation of intact porcine AC. Furthermore, biochemical assessments supported some of these findings and changed significantly after incubating the cartilage matrix for 36-72 h in PBS in terms of chondroitin sulfate and hydroxyproline content. [Reprinted with permission from Elsevier. Copyright, 2006].

Details

  • Original title: Cryopreservation of porcine articular cartilage: MRI and biochemical results after different freezing protocols.
  • Record ID : 2007-1308
  • Languages: English
  • Source: Cryobiology - vol. 54 - n. 1
  • Publication date: 2007/02

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