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Cryoconservation de semence chez les animaux domestiques : synthèse des défis actuels, des applications et des stratégies prospectives

Cryopreservation of semen in domestic animals: a review of current challenges, applications, and prospective strategies.

Auteurs : SHARAFI M., BORGHEI-RAD S. M., HEZAVEHEI M., SHAHVERDI A., BENSON J. D.

Type d'article : Article de périodique, Synthèse

Résumé

Cryopreservation is a way to preserve germplasm with applications in agriculture, biotechnology, and conservation of endangered animals. Cryopreservation has been available for over a century, yet, using current methods, only around 50% of spermatozoa retain their viability after cryopreservation. This loss is associated with damage to different sperm components including the plasma membrane, nucleus, mitochondria, proteins, mRNAs, and microRNAs. To mitigate this damage, conventional strategies use chemical additives that include classical cryoprotectants such as glycerol, as well as antioxidants, fatty acids, sugars, amino acids, and membrane stabilizers. However, clearly current protocols do not prevent all damage. This may be due to the imperfect function of antioxidants and the probable conversion of media components to more toxic forms during cryopreservation.

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Détails

  • Titre original : Cryopreservation of semen in domestic animals: a review of current challenges, applications, and prospective strategies.
  • Identifiant de la fiche : 30031291
  • Langues : Anglais
  • Sujet : Technologie
  • Source : Animals - vol. 12 - n. 23
  • Éditeurs : MDPI
  • Date d'édition : 12/2022
  • DOI : http://dx.doi.org/https://doi.org/10.3390/ani12233271

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