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Cryoconservation des cellules en suspension et des protoplastes de carottes (Daucus carota L.) par vitrification.

Cryopreservation of carrot (Daucus carota L.) cell suspensions and protoplasts by vitrification.

Auteurs : CHEN Y., WANG J. H.

Type d'article : Article

Résumé

Carrot cell suspensions and protoplasts were successfully cryopreserved by vitrification. After loading of the precultured carrot cell at room temperature for 5 min and treatment for 7.5 min, they were quenched in liquid nitrogen. Optimal survival was 83.3% following warming and unloading. Recovered cells retained the ability to regenerate plantlets in vitro. In the case of vitrification of protoplasts isolated from carrot cell suspensions, the optimal loading and dehydration durations were 5 min in 25% PVS2 and 3 min 100% in PVS2 respectively. Survival of 47% of the untreated control was achieved after cryopreservation.

Détails

  • Titre original : Cryopreservation of carrot (Daucus carota L.) cell suspensions and protoplasts by vitrification.
  • Identifiant de la fiche : 2003-3039
  • Langues : Anglais
  • Source : CryoLetters - vol. 24 - n. 1
  • Date d'édition : 01/2003
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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