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Influence des traitements avant mise en culture sur la récupération après décongélation des cultures cellulaires de topinambours (Helianthus tuberosus L.).

Influence of preculture conditions on the post-thaw recovery of suspension cultures of Jerusalem artichoke (Helianthus tuberosus L.).

Auteurs : SWAN T. W., O'HARE D., GILL R. A., et al.

Type d'article : Article

Résumé

Cells of Helianthus tuberius have been successfully recovered after controlled rate freezing, but post-thaw growth was dependent upon the preculture treatment. Mannitol preculture treatments (24 h culture in medium containing 0.5 or 0.75 M mannitol prior to freezing) resulted in significant reductions in prefreeze cell growth and cell viability, as determined by triphenyl tetrazolium chloride (TTC) reduction. No post-thaw cell growth occurred after mannitol preculture treatments. However, the use of sucrose at the same concentrations during preculture, resulted in greater prefreeze cell growth, TTC values and post-thaw cell growth. After preculture with 0.5 M sucrose, sufficient cell growth occurred by 28 days after thawing to allow re-establishment of suspension cultures.

Détails

  • Titre original : Influence of preculture conditions on the post-thaw recovery of suspension cultures of Jerusalem artichoke (Helianthus tuberosus L.).
  • Identifiant de la fiche : 2000-2797
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 20 - n. 5
  • Date d'édition : 09/1999
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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