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Cryoconservation des graines d'orchidées thaïlandaises (Doritis pulcherrima lindl.) par vitrification.

Cryopreservation of seeds of a Thai orchid (Doritis pulcherrima lindl.) by vitrification.

Auteurs : THAMMASIRI K.

Type d'article : Article

Résumé

Seeds from selfing of a Thai orchid (Doritis pulcherrima Lindl.) were successfully cryopreserved in liquid nitrogen (LN) using the vitrification method. Seeds from 3-month-old pods were sufficiently dehydrated in 2 ml cryotubes filled with highly concentrated vitrification solution (PVS2) at 25 plus or minus 2 °C for 50 min. The seeds were then rapidly plunged into LN. After rapid warming, the PVS2 solution was replaced with 0.5 ml of 1.2 M sucrose in modified Vacin and Went (VW) solution and kept at 25 plus or minus 2 °C for 20 min prior to transfer on VW agar medium. About 62% of cryopreserved seeds treated with PVS2 solution were able to develop into normal seedlings while without that treatment there was no survival. This vitrification protocol appears promising.

Détails

  • Titre original : Cryopreservation of seeds of a Thai orchid (Doritis pulcherrima lindl.) by vitrification.
  • Identifiant de la fiche : 2001-2512
  • Langues : Anglais
  • Source : CryoLetters - vol. 21 - n. 4
  • Date d'édition : 07/2000
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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