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Cryopreservation of seeds of a Thai orchid (Doritis pulcherrima lindl.) by vitrification.

Author(s) : THAMMASIRI K.

Type of article: Article

Summary

Seeds from selfing of a Thai orchid (Doritis pulcherrima Lindl.) were successfully cryopreserved in liquid nitrogen (LN) using the vitrification method. Seeds from 3-month-old pods were sufficiently dehydrated in 2 ml cryotubes filled with highly concentrated vitrification solution (PVS2) at 25 plus or minus 2 °C for 50 min. The seeds were then rapidly plunged into LN. After rapid warming, the PVS2 solution was replaced with 0.5 ml of 1.2 M sucrose in modified Vacin and Went (VW) solution and kept at 25 plus or minus 2 °C for 20 min prior to transfer on VW agar medium. About 62% of cryopreserved seeds treated with PVS2 solution were able to develop into normal seedlings while without that treatment there was no survival. This vitrification protocol appears promising.

Details

  • Original title: Cryopreservation of seeds of a Thai orchid (Doritis pulcherrima lindl.) by vitrification.
  • Record ID : 2001-2512
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 4
  • Publication date: 2000/07
  • Document available for consultation in the library of the IIR headquarters only.

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