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Cryoconservation des ovocytes de boeuf : impact du stade méiotique, du traitement au cycloheximide et de la méthode de vitrification.

Cryopreservation of cattle oocytes: effects of meiotic stage, cycloheximide treatment and vitrification procedure.

Auteurs : LE GAL F., MASSIP A.

Type d'article : Article

Résumé

Different parameters likely to influence the survival of bovine oocytes after a vitrification procedure were evaluated: oocyte meiotic stage, cycloheximide treatment at the beginning or the end of maturation, and three vitrification procedures using conventional straws, open pulled straws (OPS), or microdrops. Results show that cryoprotectant exposure reduced embryo development and that cycloheximide treatment had no beneficial effect on oocytes vitrified in conventional straws. Among the three vitrification procedures, only the OPS method yielded blastocysts (approximately 3% of vitrified oocytes) irrespective of their initial meiotic stage. This result highlights the major influence of the cooling rate in an oocyte vitrification protocol.

Détails

  • Titre original : Cryopreservation of cattle oocytes: effects of meiotic stage, cycloheximide treatment and vitrification procedure.
  • Identifiant de la fiche : 2000-2225
  • Langues : Anglais
  • Source : Cryobiology - vol. 38 - n. 4
  • Date d'édition : 06/1999

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