Partager

Document IIF

Effet de la communication cellule à cellule de l'écart de jonction sur la propagation de la glace intracellulaire et sur la viabilité cellulaire correspondante.

Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability.

Numéro : pap. n. 437

Auteurs : FUKUNAGA T., KUBO H., KURATA K., et al.

Résumé

Intracellular ice formation (IIF) propagates from cell to cell and causes lethal damage to the cell during the freezing process. In this study, we hypothesized that IIF passes through gap junctions between cells and that blockage of gap junctions increases cell viability after the freeze–thaw process because of the inhibited IIF propagation. Monolayer cultures of Madin–Darby canine kidney (MDCK) cells on glass coverslips were preincubated with and without the gap-junction blocker heptanol and subsequently frozen at different cooling rates on a temperature-controlled stage. Microscopic observations showed that IIF propagation was successfully inhibited in the blocked group. However, cell viability in the blocked group after thawing was significantly lower than that in the non-blocked group. Although results of this study did not confirm our hypotheses, they indicated the important role of gap-junctional communication in the occurrence of IIF and the consequent cell damage.

Documents disponibles

Format PDF

Pages : 7 p.

Disponible

  • Prix public

    20 €

  • Prix membre*

    Gratuit

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails

  • Titre original : Effect of gap-junctional cell-to-cell communication on intracellular ice propagation and consequent cell viability.
  • Identifiant de la fiche : 30015855
  • Langues : Anglais
  • Source : Proceedings of the 24th IIR International Congress of Refrigeration: Yokohama, Japan, August 16-22, 2015.
  • Date d'édition : 16/08/2015
  • DOI : http://dx.doi.org/10.18462/iir.icr.2015.0437

Liens


Voir d'autres communications du même compte rendu (657)
Voir le compte rendu de la conférence

Indexation