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The effect of cryopreservation on the expression of stemness-genes in mouse fetal liver cells.

Number: pap. n. 62

Author(s) : BORYSOV P., DIMITROV A., OSTANKOV M., et al.

Summary

Nowadays mesenchymal (MSCs) and hematopoetic (HSCs) stem cells of fetal liver (FL) are used in cell and tissue therapy. The important part of application of biological objects in clinical practice is cryopreservation. Stem cells self-renewal and pluripotency are controlled by transcription factors. The most famous are Nanog, Oct4 and SOX2. The analysis of stemness-genes (nanog, oct4 and sox2) state could give understanding of principles of cell genomic apparatus functioning after cryopreservation, and upgrade existing cryopreservation protocols. It has been demonstrated that our methods of isolation and cryopreservation allow saving high percentage of viable cells. The expression level of investigated genes in FL suspension and isolated MSCs and HSCs was different before and after cryopreservation. Thus, cryopreservation changes pattern of stemness-genes expression in studied fractions, which can affect their features in different ways.

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Details

  • Original title: The effect of cryopreservation on the expression of stemness-genes in mouse fetal liver cells.
  • Record ID : 30011194
  • Languages: English
  • Source: Cryogenics 2014. Proceedings of the 13th IIR International Conference: Prague, Czech Republic, April 7-11, 2014.
  • Publication date: 2014/04/07

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