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Effet du stress osmotique sur l'aptitude à la déshydratation et à la cryoconservation des pousses de Solanum tuberosum.

Effect of osmotic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips.

Auteurs : GROSPIETSCH M., STODULKOVÁ E., ZÁMECNÍK J.

Type d'article : Article

Résumé

Four different preculture treatments were tested for their ability to enhance survival of potato shoot tips (cv. Desirée) after both dehydration and cryopreservation. An encapsulation/dehydration method and rapid freezing were used. Accumulation of sucrose, glucose, fructose, glycerol and proline in the apices during treatments was measured. Very high internal sucrose concentration (up to 27.8% of dry mass) was essential to reach high survival rates after cryopreservation, but not after dehydration only. The highest survival rate (78.8%) was obtained when osmotic stress was induced by adding 2 M sucrose solution to donor plants for 5 days, followed by 0.7 M sucrose preculture of isolated shoot tips for 1 day.

Détails

  • Titre original : Effect of osmotic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips.
  • Identifiant de la fiche : 2000-3212
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 20 - n. 6
  • Date d'édition : 11/1999
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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