Share

Effect of osmotic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips.

Author(s) : GROSPIETSCH M., STODULKOVÁ E., ZÁMECNÍK J.

Type of article: Article

Summary

Four different preculture treatments were tested for their ability to enhance survival of potato shoot tips (cv. Desirée) after both dehydration and cryopreservation. An encapsulation/dehydration method and rapid freezing were used. Accumulation of sucrose, glucose, fructose, glycerol and proline in the apices during treatments was measured. Very high internal sucrose concentration (up to 27.8% of dry mass) was essential to reach high survival rates after cryopreservation, but not after dehydration only. The highest survival rate (78.8%) was obtained when osmotic stress was induced by adding 2 M sucrose solution to donor plants for 5 days, followed by 0.7 M sucrose preculture of isolated shoot tips for 1 day.

Details

  • Original title: Effect of osmotic stress on the dehydration tolerance and cryopreservation of Solanum tuberosum shoot tips.
  • Record ID : 2000-3212
  • Languages: English
  • Source: Cryo-Letters - vol. 20 - n. 6
  • Publication date: 1999/11
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (2)
See the source

Indexing