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Entreposage sous des conditions hypothermiques d'hépatocytes isolés : comparaison d'une solution à base de sucrose et d'autres solutions cryoprotectrices.

A comparison of a sucrose-based solution with other preservation media for cold storage of isolated hepatocytes.

Auteurs : SHANINA I. V., KRAVCHENKO L. P., FULLER B. J., et al.

Type d'article : Article

Résumé

A sucrose-based solution has been compared with other preservation solutions (University of Wisconsin solution and Marshall's citrate solution, with Dulbecco's medium as control) during hypothermic preservation of isolated rat hepatocytes for up to 72 h. Studies on the stability of liver cells at low temperature by exclusion of trypan blue dye and morphological appearance were conducted. These data suggest that sucrose-based solution should be investigated further as a less complex alternative solution for storage of isolated hepatocytes.

Détails

  • Titre original : A comparison of a sucrose-based solution with other preservation media for cold storage of isolated hepatocytes.
  • Identifiant de la fiche : 2002-1639
  • Langues : Anglais
  • Source : Cryobiology - vol. 41 - n. 4
  • Date d'édition : 12/2000

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