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Etude des réponses osmotiques des suspensions de cellules : comparaison des mesures optiques et électriques.

Comparison of optical measurement and electrical measurement techniques for the study of osmotic responses of cell suspensions.

Auteurs : ACKER J. P., PASCH J., HESCHEL I., et al.

Type d'article : Article

Résumé

An understanding of the osmotic response of cells during freezing and thawing is critical to the development of successful cryopreservation protocols; calculation of osmotic parameters requires a detailed description of the cell volume. Two common ways to measure volume changes are to use optical measurement techniques or an electronic particle counter. The purpose of this investigation was to compare the effectiveness of these two techniques in measuring the osmotic response of human keratinocytes that were exposed to hypo- and hypertonic salt solutions. The osmotic parameters calculated using the two methods were not significantly different. Both the optical measurement method and the electronic particle counter have inherent advantages and disadvantages that must be considered when conducting osmotic studies.

Détails

  • Titre original : Comparison of optical measurement and electrical measurement techniques for the study of osmotic responses of cell suspensions.
  • Identifiant de la fiche : 2000-2798
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 20 - n. 5
  • Date d'édition : 09/1999
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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