Partager

Étude expérimentale de la vitesse de refroidissement d’un nouveau type de support de congélation d’ovocytes.

[In Chinese. / En chinois.]

Auteurs : LI W., WANG L., LIU B., et al.

Type d'article : Article

Résumé

Cooling rate is the key factor that determines the cryopreserving effect of cells, which has much to do with the forms and the usages of freezing carrier. In this study, digital oscilloscopes and type T thermocouple of 80 µm diameter were employed to establish a high speed temperature measurement system. The cooling rates of cells were measured by using a new type of enclosed oocyte freezing carrier in different ways of being inserted from air to liquid nitrogen. The results showed that the cooling of freezing carrier is mainly fulfilled via heat convection; when the carriers were horizontally placed, the cooling rate of carrier when the air hole was perpendicular to liquid nitrogen is higher than that when the air hole was tangent to liquid nitrogen; when the freezing carrier was directly inserted into liquid nitrogen, the cooling rate of carrier was higher than that when the freezing carrier was inserted into liquid nitrogen and then swayed; when the freezing carrier was placed downwards, the cooling rate of carrier was higher than that vertically or horizontally placed. Based on the results mentioned above, when the carrier was directly inserted into liquid nitrogen downwards, the cooling rate could reach as high as 54572 ± 1338 K/min, which is the highest and favourable to verification of oocytes.

Documents disponibles

Format PDF

Pages : 114-118

Disponible

  • Prix public

    20 €

  • Prix membre*

    15 €

* meilleur tarif applicable selon le type d'adhésion (voir le détail des avantages des adhésions individuelles et collectives)

Détails


Liens


Voir d'autres articles du même numéro (3)
Voir la source

Indexation