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Experimental study on the cooling rate of a new type of oocyte carriers.

[In Chinese. / En chinois.]

Author(s) : LI W., WANG L., LIU B., et al.

Type of article: Article

Summary

Cooling rate is the key factor that determines the cryopreserving effect of cells, which has much to do with the forms and the usages of freezing carrier. In this study, digital oscilloscopes and type T thermocouple of 80 µm diameter were employed to establish a high speed temperature measurement system. The cooling rates of cells were measured by using a new type of enclosed oocyte freezing carrier in different ways of being inserted from air to liquid nitrogen. The results showed that the cooling of freezing carrier is mainly fulfilled via heat convection; when the carriers were horizontally placed, the cooling rate of carrier when the air hole was perpendicular to liquid nitrogen is higher than that when the air hole was tangent to liquid nitrogen; when the freezing carrier was directly inserted into liquid nitrogen, the cooling rate of carrier was higher than that when the freezing carrier was inserted into liquid nitrogen and then swayed; when the freezing carrier was placed downwards, the cooling rate of carrier was higher than that vertically or horizontally placed. Based on the results mentioned above, when the carrier was directly inserted into liquid nitrogen downwards, the cooling rate could reach as high as 54572 ± 1338 K/min, which is the highest and favourable to verification of oocytes.

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Pages: 114-118

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