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Facteurs influençant la survie d'embryons d'huîtres (Crassostrea gigas) cryoconservés.

Factors affecting survival of cryopreserved oyster (Crassostrea gigas) embryos.

Auteurs : LIN T. T., CHAO N. H., TUNG H. T.

Type d'article : Article

Résumé

A two-step freezing procedure was developed and optimized in order to cryopreserve oyster embryos. The effects of cooling rate, choice of cryoprotectant, and seeding temperature on the survival of late-stage oyster embryos were examined. When these factors were optimized, improved survival rates of 78 more or less 8% and 83 more or less 7% were achieved using 2 M DMSO or glycerol, respectively, as cryoprotectant. Experimental results indicate that oyster embryos survive after freezing over a broad range of cooling rates ranging from -0.5 to -16 °C/min. DMSO, glycerol, propylene glycol, and ethylene glycol may be used as cryoprotectants for the cryopreservation of oyster embryos.

Détails

  • Titre original : Factors affecting survival of cryopreserved oyster (Crassostrea gigas) embryos.
  • Identifiant de la fiche : 2001-0548
  • Langues : Anglais
  • Source : Cryobiology - vol. 39 - n. 2
  • Date d'édition : 09/1999

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