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Factors affecting survival of cryopreserved oyster (Crassostrea gigas) embryos.

Author(s) : LIN T. T., CHAO N. H., TUNG H. T.

Type of article: Article

Summary

A two-step freezing procedure was developed and optimized in order to cryopreserve oyster embryos. The effects of cooling rate, choice of cryoprotectant, and seeding temperature on the survival of late-stage oyster embryos were examined. When these factors were optimized, improved survival rates of 78 more or less 8% and 83 more or less 7% were achieved using 2 M DMSO or glycerol, respectively, as cryoprotectant. Experimental results indicate that oyster embryos survive after freezing over a broad range of cooling rates ranging from -0.5 to -16 °C/min. DMSO, glycerol, propylene glycol, and ethylene glycol may be used as cryoprotectants for the cryopreservation of oyster embryos.

Details

  • Original title: Factors affecting survival of cryopreserved oyster (Crassostrea gigas) embryos.
  • Record ID : 2001-0548
  • Languages: English
  • Source: Cryobiology - vol. 39 - n. 2
  • Publication date: 1999/09

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