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La séquence utilisée pour la préparation a une incidence sur le succès de la cryoconservation des embryons somatiques de manioc.

The sequence of the preparative procedures affects the success of cryostorage of cassava somatic embryos.

Auteurs : STEWART P., TAYLOR M., MYCOCK D.

Type d'article : Article

Résumé

Cassava primary somatic embryos were prepared for cryostorage using both physical drying and cryoprotectant application stages. The effects of the sequence by which these preparative events were applied to the material were investigated. The drying of the somatic embryos before the application of the cryoprotectants resulted in a significantly higher viability after cryostorage than when the material was first exposed to the cryoprotectants then dried. The impact of these preparative procedures on sub-cellular detail was monitored by means of transmission electron microscopy.

Détails

  • Titre original : The sequence of the preparative procedures affects the success of cryostorage of cassava somatic embryos.
  • Identifiant de la fiche : 2002-0209
  • Langues : Anglais
  • Source : CryoLetters - vol. 22 - n. 1
  • Date d'édition : 01/2001
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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