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The sequence of the preparative procedures affects the success of cryostorage of cassava somatic embryos.

Author(s) : STEWART P., TAYLOR M., MYCOCK D.

Type of article: Article

Summary

Cassava primary somatic embryos were prepared for cryostorage using both physical drying and cryoprotectant application stages. The effects of the sequence by which these preparative events were applied to the material were investigated. The drying of the somatic embryos before the application of the cryoprotectants resulted in a significantly higher viability after cryostorage than when the material was first exposed to the cryoprotectants then dried. The impact of these preparative procedures on sub-cellular detail was monitored by means of transmission electron microscopy.

Details

  • Original title: The sequence of the preparative procedures affects the success of cryostorage of cassava somatic embryos.
  • Record ID : 2002-0209
  • Languages: English
  • Source: CryoLetters - vol. 22 - n. 1
  • Publication date: 2001/01
  • Document available for consultation in the library of the IIR headquarters only.

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