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Purification et caractérisation partielle des protéines de l'hystérésis thermique des larves des insectes parasitaires des aiguilles de pin Thecodiplosis japonensis (Diptera: cecidomiidae).

Purification and partial characterization of thermal hysteresis proteins from overwintering larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera: cecidomiidae).

Auteurs : LI Y., GONG H., PARK H. Y.

Type d'article : Article

Résumé

The pine needle gall midge of Thecodiplosis japonensis is a serious forest pest and overwinters as a 3rd instar larva at soil surface in Korea. The time necessary for killing 50% of larvae at -15 °C is 160 min. During overwintering period, T. japonensis larvae accumulate a relatively high content of trehalose as the main cryoprotectant. In this paper, the proteinaceous cryoprotectants were identified. Two thermal hysteresis proteins (THP-1S and 2S) were purified from overwintering larvae by ethanol fractionation, trichloroacetic acid precipitation, purified from overwintering larvae by ethanol fractionation, trichloroacetic acid precipitation, ion-exchange chromatography (DEAE-Sephadex A-25) and gel permeation chromatography (Sephadex G-100). T. japonensis THPs cannot be stained by periodic acid-Schiffs' reagent, suggesting no carbohydrate in them. The thermal hysteresis activity of THP-2 at the concentration of 50 mg/ml is 11.02 plus or minus 0.08 °C, perhaps the highest active insect THP. This is the first report of purified T. japonensis THPs in Diptera.

Détails

  • Titre original : Purification and partial characterization of thermal hysteresis proteins from overwintering larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera: cecidomiidae).
  • Identifiant de la fiche : 2001-1404
  • Langues : Anglais
  • Source : CryoLetters - vol. 21 - n. 2
  • Date d'édition : 03/2000
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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