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Purification and partial characterization of thermal hysteresis proteins from overwintering larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera: cecidomiidae).

Author(s) : LI Y., GONG H., PARK H. Y.

Type of article: Article

Summary

The pine needle gall midge of Thecodiplosis japonensis is a serious forest pest and overwinters as a 3rd instar larva at soil surface in Korea. The time necessary for killing 50% of larvae at -15 °C is 160 min. During overwintering period, T. japonensis larvae accumulate a relatively high content of trehalose as the main cryoprotectant. In this paper, the proteinaceous cryoprotectants were identified. Two thermal hysteresis proteins (THP-1S and 2S) were purified from overwintering larvae by ethanol fractionation, trichloroacetic acid precipitation, purified from overwintering larvae by ethanol fractionation, trichloroacetic acid precipitation, ion-exchange chromatography (DEAE-Sephadex A-25) and gel permeation chromatography (Sephadex G-100). T. japonensis THPs cannot be stained by periodic acid-Schiffs' reagent, suggesting no carbohydrate in them. The thermal hysteresis activity of THP-2 at the concentration of 50 mg/ml is 11.02 plus or minus 0.08 °C, perhaps the highest active insect THP. This is the first report of purified T. japonensis THPs in Diptera.

Details

  • Original title: Purification and partial characterization of thermal hysteresis proteins from overwintering larvae of pine needle gall midge, Thecodiplosis japonensis (Diptera: cecidomiidae).
  • Record ID : 2001-1404
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 2
  • Publication date: 2000/03
  • Document available for consultation in the library of the IIR headquarters only.

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