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Utilisation de la glace carbonique pour la congélation sous forme de pastilles d'embryons bovins produits in vitro.

Pellet freezing of in vitro produced bovine embryos using dry ice.

Auteurs : OTOI T., ABDOON A. S. S., OMAIMA M. T. K., et al.

Type d'article : Article

Résumé

In vitro produced bovine embryos were frozen by pellet freezing or vitrification method. Using the former, the embryos were cooled on the dry ice and then frozen as pellets. At warming, the pellets were immersed directly into 0.5 M sucrose. The survival rates of blastocysts frozen by the pellet freezing method were higher (P lower than 0.01) in 40% ethylene glycol (EG) than those in the lower concentrations (20 and 30% EG). Higher survival rates of blastocysts frozen by the pellet freezing method were obtained but the development rates did not differ, as compared with those by the vitrification method. These results demonstrate that the pellet freezing method using dry ice can be used successfully for the cryopreservation of blastocysts.

Détails

  • Titre original : Pellet freezing of in vitro produced bovine embryos using dry ice.
  • Identifiant de la fiche : 2001-1092
  • Langues : Anglais
  • Source : CryoLetters - vol. 21 - n. 1
  • Date d'édition : 01/2000
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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