Share

Pellet freezing of in vitro produced bovine embryos using dry ice.

Author(s) : OTOI T., ABDOON A. S. S., OMAIMA M. T. K., et al.

Type of article: Article

Summary

In vitro produced bovine embryos were frozen by pellet freezing or vitrification method. Using the former, the embryos were cooled on the dry ice and then frozen as pellets. At warming, the pellets were immersed directly into 0.5 M sucrose. The survival rates of blastocysts frozen by the pellet freezing method were higher (P lower than 0.01) in 40% ethylene glycol (EG) than those in the lower concentrations (20 and 30% EG). Higher survival rates of blastocysts frozen by the pellet freezing method were obtained but the development rates did not differ, as compared with those by the vitrification method. These results demonstrate that the pellet freezing method using dry ice can be used successfully for the cryopreservation of blastocysts.

Details

  • Original title: Pellet freezing of in vitro produced bovine embryos using dry ice.
  • Record ID : 2001-1092
  • Languages: English
  • Source: CryoLetters - vol. 21 - n. 1
  • Publication date: 2000/01
  • Document available for consultation in the library of the IIR headquarters only.

Links


See other articles in this issue (3)
See the source

Indexing