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Cryoconservation des axes embryonnaires de Citrus madurensis par vitrification : importance de la charge en solution de vitrification.

Cryopreservation of Citrus madurensis embryonic axes by vitrification: importance of loading and treatment with vitrification solution.

Auteurs : CHO E. G., HOR Y. L., KIM H. H., et al.

Type d'article : Article

Résumé

The authors investigated the importance of loading and treatment with a vitrification solution on the survival of Citrus madurensis embryonic axes cryopreserved using a vitrification protocol. Among the seven different loading solutions tested, that containing 2 M glycerol + 0.4 M sucrose was the most efficient. Of the six vitrification solutions tested, the PVS2 vitrification solution ensured the highest survival. A 3-step vitrification protocol, involving the treatment of embryonic axes at 0 °C with half strength PVS2 solution for 20 min then with full strength PVS2 for an additional 40 min was more efficient than a 2-step protocol involving treatment of axes directly with full strength PVS2 solution for 60 min. After rapid immersion in liquid nitrogen, rapid rewarming, unloading in a 1.2 M sucrose solution for 20 min, culture on solid medium with 0.3 M sucrose for 1 day and growth recovery for 4 weeks on standard medium, survival of Citrus madurensis embryonic axes reached 85% following the 3-step process, compared with 70% for the 2-step process.

Détails

  • Titre original : Cryopreservation of Citrus madurensis embryonic axes by vitrification: importance of loading and treatment with vitrification solution.
  • Identifiant de la fiche : 2003-3028
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 5
  • Date d'édition : 09/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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