Partager

Cryoconservation des axes embryonnaires de Citrus madurensis par encapsulation-déshydratation.

Cryopreservation of Citrus madurensis embryonic axes by encapsulation-dehydration.

Auteurs : CHO E. G., HOR Y. L., KIM H. H., et al.

Type d'article : Article

Résumé

The authors demonstrated that Citrus madurensis embryonic axes can withstand cryopreservation using the encapsulation-dehydration technique. Up to 57.5% survival was achieved using a standard encapsulation-dehydration protocol, which included pregrowth of encapsulated axes for 16 h in medium containing 0.8 M sucrose + 1 M glycerol, desiccation of beads to around 30% moisture content followed by rapid freezing. A slightly higher survival percentage (65%) was obtained using a modified encapsulation-dehydration protocol, which included pretreatment of axes with 2 M glycerol + 0.6 M sucrose for 1 h, concomitantly with their encapsulation in 3% calcium alginate beads, followed by desiccation of the beads to around 30% moisture content.

Détails

  • Titre original : Cryopreservation of Citrus madurensis embryonic axes by encapsulation-dehydration.
  • Identifiant de la fiche : 2003-3027
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 5
  • Date d'édition : 09/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

Liens


Voir d'autres articles du même numéro (3)
Voir la source

Indexation