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Cryopreservation of Citrus madurensis embryonic axes by vitrification: importance of loading and treatment with vitrification solution.

Author(s) : CHO E. G., HOR Y. L., KIM H. H., et al.

Type of article: Article

Summary

The authors investigated the importance of loading and treatment with a vitrification solution on the survival of Citrus madurensis embryonic axes cryopreserved using a vitrification protocol. Among the seven different loading solutions tested, that containing 2 M glycerol + 0.4 M sucrose was the most efficient. Of the six vitrification solutions tested, the PVS2 vitrification solution ensured the highest survival. A 3-step vitrification protocol, involving the treatment of embryonic axes at 0 °C with half strength PVS2 solution for 20 min then with full strength PVS2 for an additional 40 min was more efficient than a 2-step protocol involving treatment of axes directly with full strength PVS2 solution for 60 min. After rapid immersion in liquid nitrogen, rapid rewarming, unloading in a 1.2 M sucrose solution for 20 min, culture on solid medium with 0.3 M sucrose for 1 day and growth recovery for 4 weeks on standard medium, survival of Citrus madurensis embryonic axes reached 85% following the 3-step process, compared with 70% for the 2-step process.

Details

  • Original title: Cryopreservation of Citrus madurensis embryonic axes by vitrification: importance of loading and treatment with vitrification solution.
  • Record ID : 2003-3028
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 5
  • Publication date: 2002/09
  • Document available for consultation in the library of the IIR headquarters only.

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