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Cryoconservation des cellules de Papaver somniferum modifiées.

Cryopreservation of transformed Papaver somniferum cells.

Auteurs : GAZEAU C., ELLEUCH H., DAVID A., MORISSET C.

Type d'article : Article

Résumé

Different procedures were tested in an attempt to cryopreserve transformed cells of Papaver somniferum in liquid nitrogen. The encapsulation-dehydration process yielded negative results. The vitrification method also proved ineffective. In contrast, using the conventional procedure, suspension cultures resisted to -196 deg C, when treated with 0.75 M sorbitol or maltose and 5% DMSO, and two-step frozen. Better results after freezing were achieved using an original method including encapsulation of cells and conventional cryopreservation. This procedure based on trapping of cells in calcium-alginate allowed easier handling, faster regrowth than for non-encapsulated cells, and sucessful cryopreservation at -196 deg C of Papaver suspensions after a cryoprotective treatment combined with progressive freezing.

Détails

  • Titre original : Cryopreservation of transformed Papaver somniferum cells.
  • Identifiant de la fiche : 1999-1936
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 19 - n. 3
  • Date d'édition : 05/1998
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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