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Cryoconservation des cellules souches dans des banques de sang ombilical et de moelle épinière.

[In Russian. / En russe.]

Auteurs : SMOLÂNINOV A. B., KOVAN'KO G. N., BAGAUTDINOV S. N., et al.

Type d'article : Article

Résumé

The nature of destruction of a cell during its freezing and the mechanism of prevention of this destruction using cryoprotectants has been considered. Special features of cryopreservation of founder cells (FC) and the technique of this process are described. A container for the storage of founder cells and methods of maintaining low temperatures during storage of FC: nitrogen vapour or liquid nitrogen chilling, and also mechanical chilling are described. Cryoprotectants for cryopreservation of FC: glycerol, dimethylsulfoxide, hydroxyethylstarch and other polymers are described in detail. Recommendations on chilling conditions of FC during their cryopreservation and heating before use given.

Détails

  • Titre original : [In Russian. / En russe.]
  • Identifiant de la fiche : 2010-0279
  • Langues : Russe
  • Source : Vestnik Mezdunarodarnoj Akademii Holoda - n. 2
  • Date d'édition : 2009

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