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Cryopreservation of transformed Papaver somniferum cells.

Author(s) : GAZEAU C., ELLEUCH H., DAVID A., MORISSET C.

Type of article: Article

Summary

Different procedures were tested in an attempt to cryopreserve transformed cells of Papaver somniferum in liquid nitrogen. The encapsulation-dehydration process yielded negative results. The vitrification method also proved ineffective. In contrast, using the conventional procedure, suspension cultures resisted to -196 deg C, when treated with 0.75 M sorbitol or maltose and 5% DMSO, and two-step frozen. Better results after freezing were achieved using an original method including encapsulation of cells and conventional cryopreservation. This procedure based on trapping of cells in calcium-alginate allowed easier handling, faster regrowth than for non-encapsulated cells, and sucessful cryopreservation at -196 deg C of Papaver suspensions after a cryoprotective treatment combined with progressive freezing.

Details

  • Original title: Cryopreservation of transformed Papaver somniferum cells.
  • Record ID : 1999-1936
  • Languages: English
  • Source: Cryo-Letters - vol. 19 - n. 3
  • Publication date: 1998/05
  • Document available for consultation in the library of the IIR headquarters only.

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