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Cryoconservation des spermatozoïdes de daurade royale (Sparus aurata).

Cryopreservation of seabream (Sparus aurata) spermatozoa.

Auteurs : FABBROCINI S., LAVADERA S. L., RISPOLI S., et al.

Type d'article : Article

Résumé

The aim of this research was to optimize protocols for freezing spermatozoa of seabream (Sparus aurata). All the phrases of the cryopreservation procedure (sampling, choosing the cryoprotective extender, cooling, freezing and thawing) were studied in relation to the species of spermatozoa under examination, so as to be able to restore on thawing the morphological and physiological characteristics of fresh semen. Five cryoprotectants, dimethyl sulfoxide (DMSO), ethylene glycol (EG), 1,2-propylene glycol (PG), glycerol, and methanol, were tested at concentrations between five and 15% by volume to evaluate their effect on the motility of semen exposed for up to 30 min at 26 °C. On thawing, the best motility was obtained with 5% DMSO, although both 10% PG and EG showed good results; no differences were found between the two freezing gradients, although semen frozen with the 10 °C/min gradient showed a slightly higher and more prolonged motility.

Détails

  • Titre original : Cryopreservation of seabream (Sparus aurata) spermatozoa.
  • Identifiant de la fiche : 2001-1094
  • Langues : Anglais
  • Source : Cryobiology - vol. 40 - n. 1
  • Date d'édition : 02/2000

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