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Effets des procédures de cryoconservation sur la viabilité génotypique des cellules de riz.

Effects of the cryopreservation procedures on recovered rice cell populations.

Auteurs : MOUKADIRI O., O'CONNOR J. E., CORNEJO M. J.

Type d'article : Article

Résumé

Cryogenic storage of plant cells allows the long-term maintenance of valuable genotypes. Rice calli (Oryza sativa L.) were cryopreserved using cryoprotectants and slow cooling rates, as well as direct immersion in liquid nitrogen without cryoprotection. Subsequently, the characteristics of the recovered cells as well as the effects of putative cryoselection were investigated by microscopic observations and flow cytometric analyses. For this purpose, protoplasts were prepared from calli that had been cryopreserved by direct plunging into liquid nitrogen and from their unfrozen controls. Results show that direct immersion in liquid nitrogen and treated with abscisic acid is a fast and highly efficient freezing procedure that maintains the main characteristics of the cell populations and appears to increase their metabolic activity.

Détails

  • Titre original : Effects of the cryopreservation procedures on recovered rice cell populations.
  • Identifiant de la fiche : 2002-3204
  • Langues : Anglais
  • Source : CryoLetters - vol. 23 - n. 1
  • Date d'édition : 01/2002
  • Document disponible en consultation à la bibliothèque du siège de l'IIF uniquement.

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