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Effects of the cryopreservation procedures on recovered rice cell populations.

Author(s) : MOUKADIRI O., O'CONNOR J. E., CORNEJO M. J.

Type of article: Article

Summary

Cryogenic storage of plant cells allows the long-term maintenance of valuable genotypes. Rice calli (Oryza sativa L.) were cryopreserved using cryoprotectants and slow cooling rates, as well as direct immersion in liquid nitrogen without cryoprotection. Subsequently, the characteristics of the recovered cells as well as the effects of putative cryoselection were investigated by microscopic observations and flow cytometric analyses. For this purpose, protoplasts were prepared from calli that had been cryopreserved by direct plunging into liquid nitrogen and from their unfrozen controls. Results show that direct immersion in liquid nitrogen and treated with abscisic acid is a fast and highly efficient freezing procedure that maintains the main characteristics of the cell populations and appears to increase their metabolic activity.

Details

  • Original title: Effects of the cryopreservation procedures on recovered rice cell populations.
  • Record ID : 2002-3204
  • Languages: English
  • Source: CryoLetters - vol. 23 - n. 1
  • Publication date: 2002/01
  • Document available for consultation in the library of the IIR headquarters only.

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