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Influence of preculture conditions on the post-thaw recovery of suspension cultures of Jerusalem artichoke (Helianthus tuberosus L.).

Author(s) : SWAN T. W., O'HARE D., GILL R. A., et al.

Type of article: Article

Summary

Cells of Helianthus tuberius have been successfully recovered after controlled rate freezing, but post-thaw growth was dependent upon the preculture treatment. Mannitol preculture treatments (24 h culture in medium containing 0.5 or 0.75 M mannitol prior to freezing) resulted in significant reductions in prefreeze cell growth and cell viability, as determined by triphenyl tetrazolium chloride (TTC) reduction. No post-thaw cell growth occurred after mannitol preculture treatments. However, the use of sucrose at the same concentrations during preculture, resulted in greater prefreeze cell growth, TTC values and post-thaw cell growth. After preculture with 0.5 M sucrose, sufficient cell growth occurred by 28 days after thawing to allow re-establishment of suspension cultures.

Details

  • Original title: Influence of preculture conditions on the post-thaw recovery of suspension cultures of Jerusalem artichoke (Helianthus tuberosus L.).
  • Record ID : 2000-2797
  • Languages: English
  • Source: Cryo-Letters - vol. 20 - n. 5
  • Publication date: 1999/09
  • Document available for consultation in the library of the IIR headquarters only.

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