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The use of cryopreservation in the ex-situ conservation of fungi.

L'utilisation de la cryoconservation dans la conservation ex-situ des champignons.

Auteurs : SMITH D.

Type d'article : Article

Résumé

Of the estimated 1.5 million fungi only 72,000 strains have been described and less than 20% of these are held in collections. It is evident that many microorganisms have only been isolated once, as almost 50% of the species held by the World Data Center for Microorganisms collections are represented by one isolate only. Storage at ultra-low temperature has an essential role to play in securing these organisms for sustainable use. A two-step method of cooling was initially employed which approximated -1 deg C/ minute. Improved viability and stability was achieved by using linear cooling rates determined by cryogenic light microscopy.

Détails

  • Titre original : The use of cryopreservation in the ex-situ conservation of fungi.
  • Identifiant de la fiche : 1999-1221
  • Langues : Anglais
  • Source : Cryo-Letters - vol. 19 - n. 2
  • Date d'édition : 03/1998
  • Disponible à la bibliothèque de l'IIF

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