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The use of cryopreservation in the ex-situ conservation of fungi.

Author(s) : SMITH D.

Type of article: Article

Summary

Of the estimated 1.5 million fungi only 72,000 strains have been described and less than 20% of these are held in collections. It is evident that many microorganisms have only been isolated once, as almost 50% of the species held by the World Data Center for Microorganisms collections are represented by one isolate only. Storage at ultra-low temperature has an essential role to play in securing these organisms for sustainable use. A two-step method of cooling was initially employed which approximated -1 deg C/ minute. Improved viability and stability was achieved by using linear cooling rates determined by cryogenic light microscopy.

Details

  • Original title: The use of cryopreservation in the ex-situ conservation of fungi.
  • Record ID : 1999-1221
  • Languages: English
  • Source: Cryo-Letters - vol. 19 - n. 2
  • Publication date: 1998/03
  • Document available for consultation in the library of the IIR headquarters only.

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