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Lyophilisation d'érythrocytes à des températures ultra basses.

Freeze-drying of red blood cells at ultra-low temperatures.

Auteurs : RINDLER V., LÜNEBERGER S., SCHWINDKE P., HESCHEL I., RAU G.

Type d'article : Article

Résumé

Hemolysis of human red blood cells after freeze-drying and resuspension depends on the vacuum-drying temperature. In an experimental study, red blood cells were first solidified based on a modified high-yield cryopreservation protocol. Afterward, they were vacuum-dried in a special low-temperature freeze-drying device. Subsequently, the dried samples were resuspended in an isotonic, phosphate-buffered saline solution. Hemolysis was determined according to a modified saline stability test.

Détails

  • Titre original : Freeze-drying of red blood cells at ultra-low temperatures.
  • Identifiant de la fiche : 2000-0443
  • Langues : Anglais
  • Source : Cryobiology - vol. 38 - n. 1
  • Date d'édition : 02/1999

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