Freeze-drying of red blood cells at ultra-low temperatures.

Author(s) : RINDLER V., LÜNEBERGER S., SCHWINDKE P., HESCHEL I., RAU G.

Type of article: Article

Summary

Hemolysis of human red blood cells after freeze-drying and resuspension depends on the vacuum-drying temperature. In an experimental study, red blood cells were first solidified based on a modified high-yield cryopreservation protocol. Afterward, they were vacuum-dried in a special low-temperature freeze-drying device. Subsequently, the dried samples were resuspended in an isotonic, phosphate-buffered saline solution. Hemolysis was determined according to a modified saline stability test.

Details

  • Original title: Freeze-drying of red blood cells at ultra-low temperatures.
  • Record ID : 2000-0443
  • Languages: English
  • Source: Cryobiology - vol. 38 - n. 1
  • Publication date: 1999/02

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