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Méthodes améliorées pour la réfrigération rapide contrôlée des cellules en suspension.

Improved methods for controlled rapid cooling of cell suspensions.

Auteurs : MORRIS G. J., RICHENS H. E.

Type d'article : Article

Résumé

Simple, reproducible, methods of achieving rapid rates of cooling in the range 100 to 1000 °C/min are described for straws and cryovial. These methods use the direct contact of straws or cryovials with precooled granules or plates as the heat sink. Liquid nitrogen may be adsorbed into suitable material, for example activated charcoal, zeolites and molecular sieves as the matrix to achieve rapid cooling. Controllable rapid rates of cooling may also be attained by using non-adsorbents. The rate of cooling may be modified by changing the adsorbent material, the size of the adsorbent granules and the temperature of the adsorbent.

Détails

  • Titre original : Improved methods for controlled rapid cooling of cell suspensions.
  • Identifiant de la fiche : 2005-1500
  • Langues : Anglais
  • Source : CryoLetters - vol. 25 - n. 4
  • Date d'édition : 07/2004

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